Remove the roots from the bottle of acid using the forceps and rinse them in tap water several times and then place them in a clean petri dishÂ.       Using a pair of forceps, carefully pick one or two of the roots and place them into the bottle of warm hydrochloric acid for about 5 minutes - This serves to break down pectin and calcium pectate as well as other tissues in order to release individual cells.       Carefully pour the hydrochloric acid solution into a small bottle and place it in the water bath for about 15 minutes in order to warm the acid.This may be achieved by using a thermostatically controlled water bath        Prepare a water bath (about 55 degrees C) - The temperature should be maintained at 55 degrees C.       Using a blade or scalpel, carefully cut one or several roots from the onion and place them in a petri dish (or any other small clean container).* Having a pair of gloves, safety goggles, and safety mask is recommended given that some of the chemical used are hazardous.       Onion with freshly grown roots.      Stain - Aceto-carmine or Aceto-orcein. The following are diagrammatic representations of this setup: Let the onions rest on water for 3-4 days - only the lower surface (base from which roots emerge) should be in contact with water.In a case where the onions are too small, they can be supported using toothpick/splints.Carefully place the onion bulbs in the glass/plastic jars so that only the lower surface of the onion comes in contact with the water.Pour clean water into the clean (and clear) glass/plastic jars - about three-quarters full.* Growing fresh root tips is recommended given that older or dried roots may produce poor results. Prophase (divided into prophase and prometaphase)īecause of the rapid rate at which onion root tips grow as a result of rapid cell division, it's possible to observe and identify the different stages of mitosis.įor this section, the experiment will be divided into two main parts.In general, mitosis occurs through several stages that include: Given that the process results in the proliferation of cells, it's important for general growth and replacement of damaged cells (e.g. Metaphase in Mitosis and Meiosis – Metaphase 1 and Metaphase 2
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